The  Ag/AgCl reference electrodes serve for calibration of the working electrodes in a phosphate buffer (PB) or artificial cerebrospinal fluid (ASF) and for measurements of neurotransmitter overflow in anesthetized animals or other types of tissue preparations. These electrodes should be immersed in the PB or ASF 24 hours before measurements. The standard length of the electrode is 35 mm, diameter is 2.6 mm. However the length is customizable. The reference electrodes fit holder of the Invilog Bubble Level Probe.

Miniature Ag/AgCl electrodes (see panel below), made with PEEK (2.0 mm in diameter), glass (3.0 mm in diameter), or a PE flexible body, are filled with 3M KCl solution and can serve as replacements for standard reference electrodes in confined spaces for any experiment requiring an Ag/AgCl reference.