Equipment for in vivo voltammetry
All what one may need to set-up in vivo voltammetry laboratory, including headstages for fast cyclic voltammetry and amperometry, potentiostat, brain stimulator with stabilized current isolation unit, software designed for FSCV and amperometry and controlling stimulation, working, reference and stimulating electrodes.
From establishing of a working in vivo voltammetry laboratory and training of staff to refinement and application of the methods in preclinical pharmacology.
We arrange videoconferencing, which will take you through the entire procedure from the first calibration all the way to registration of dopamine even at nanomolar levels.
Brain stimulation and monitoring behaviour
The same animals undergoing in vivo voltammetry can be tested in some behavioral protocols without the need to register the dopamine transient but with optical or electrical stimulation of specific brain areas. We have developed several approaches that permit to perform wireless brain stimulation and to build sophisticated behavioral tests without significant investment in the new equipment.
We perform real-time neurochemical analysis of dopamine, noradrenaline, serotonin release and re-uptake in the smallest brain structures, and testing the effects of drugs and new compounds on the overflow of monoamines in different brain areas of intact mouse and rat brain.
We encapsulated Ag/AgCl reference electrode in stainless steel shield. Now it can be easily grounded. Therefore even with the longest reference electrodes (Invilog manufactures electrodes up to 20 cm in length), the level of noise will be reduced. The shield can be also used as an auxiliary electrode.
This Ag/AgCl references electrode can be installed through the wall or bottom of the experimental chamber (e.g. for slice electrophysiology) saving space above the chamber for multiple microelectrodes and their holders and decreasing wiring mess and noise.
The head stage permits fast cyclic voltammetry and electrophysiology from the same 1-30 um diameter microelectrode almost simultaneously. With this addition real-time measurements of dopamine, noradrenaline, serotonin, pH shifts, H2O2, adenosine and oxygen concentrations can be made.
Fast-scan controlled adsorption voltammetry (FSCAV), which has been described in a few recent publications, requires complicated and expensive hardware as well as its own software.
Invilog now provides an application which exploits this promising method and we are offering equipment which incorporates FSCAV as a novel component of its measurement possibilities – and at the price of our basic setup.